Quantitation of binding sites in the Tg2576 mouse brain
Images of frozen sections incubated with [3H]IN973 (examples of which are shown in Fig. 2) were analyzed using the OptiQuant image analysis software. Densitometric analysis of specific brain regions was performed and converted to femtomoles per milligram of protein based on the density of standards.
Brain Region | Mean Density | S.E.M. |
|---|---|---|
| Olfactory bulb | ||
| Glomerular layer | 245.0 | 14.7 |
| Striatum | 129.6 | 4.1 |
| Septum | 159.0 | 5.1 |
| Frontal cortex | 155.1 | 4.3 |
| Hippocampus | ||
| CA1 | 149.9 | 6.7 |
| CA2 | 169.6 | 8.2 |
| Dentate gyrus and hilus | 208.9 | 7.3 |
| Amygdaloid complex | 174.0 | 5.0 |
| Thalamus | 97.2 | 4.7 |
| Hypothalamus | ||
| Dorsomedial nucleus | 164.1 | 11.2 |
| Ventromedial nucleus | 180.9 | 14.3 |
| Pituitary | 313.1 | 36.1 |
| Substantia nigra | 180.2 | 9.0 |
| Interpeduncular nucleus | 179.7 | 19.7 |
| Central gray | 171.7 | 1.6 |
| Superior colliculus | 122.7 | 7.7 |
| Inferior colliculus | 88.5 | 6.1 |
| Cerebellum | ||
| Molecular layer | 201.6 | 13.9 |
| Choroid plexus | 269.6 | 11.4 |
| Corpus callosum | 62.5 | 4.3 |