Inhibition of platelet aggregation in platelet-rich plasma
Blood samples were freshly collected into 1/10 volume of 3.8% trisodium citrate solution. Platelet-rich plasma was prepared by centrifugation of blood samples at 150g for 10 min at room temperature. Platelet aggregation induced by 10 μM ADP was monitored at 37°C with an aggregometer in the presence or absence of test compound. Each value is the mean ± S.E.M.; n = 4.
| Compound | IC50 of Platelet Aggregation | |||
|---|---|---|---|---|
| Human | Monkey | Dog | Rat | |
| μM | ||||
| MRE-269 | 0.21 ± 0.04 | 0.21 ± 0.02 | 25 ± 1.5 | 10 ± 0.4 |
| NS-304 | 5.5 ± 0.8 | 3.4 ± 1.1 | >100 | — |
| Beraprost | 0.019 ± 0.002 | 0.025 ± 0.001 | 0.035 ± 0.004 | 0.037 ± 0.001 |
—, not tested.