TABLE 1

Ligand potencies at the wild type and mutant ghrelin receptors

Data represent the mean ± S.E.M. from at least four independent experiments using a luciferase reporter gene assay; each experiment was performed in triplicate.

Ligand	Wild Type		I134T		V160M		A204E		F279L
Ligand	EC₅₀^a	pEC₅₀ ± S.E.M.	EC₅₀^a	pEC₅₀ ± S.E.M.	EC₅₀^a	pEC₅₀ ± S.E.M.	EC₅₀^a	pEC₅₀ ± S.E.M.	EC₅₀^a	pEC₅₀ ± S.E.M.
Ghrelin	75.2	7.12 ± 0.3	No stimulation		3.8	8.42 ± 0.29^b	92.3	7.04 ± 0.13	34.9	7.36 ± 0.29
SP analog	41.8	7.38 ± 0.16	203.0	6.69 ± 0.41^b	12.1	7.92 ± 0.51	No inhibition		30.4	7.52 ± 0.60

↵ a Half-maximal effective concentration (nanomolar).
↵ b p < 0.05 versus wild-type value.