Inhibition of platelet dense granule secretion by cyclic nucleotide agonists
Vehicle | Forskolin | YC-1 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.5 μM | 2.0 μM | 4.0 μM | 10 μM | 40 μM | 80 μM | ||||||
| PAR1 | 5.2 ± 0.3 | 0.2 ± 0.1 | 0.0 | 0.0 | 1.4 ± 0.5 | 0.2 ± 0.1 | 0.0 | ||||
| PAR4 | 5.5 ± 0.6 | 3.3 ± 0.8 | 0.0 | 0.0 | 4.0 ± 0.7 | 0.0 | 0.0 | ||||
Platelets were stimulated with 2.8 μM PAR1-AP or 240 μM PAR4-AP. Values are mean micromolar ATP ± S.E. (n = 3-5).