TABLE 1

Neurite outgrowth quantification after TRO19622 treatment

The effects of low doses of TRO19622 were compared with those of optimal concentrations of neurotrophic factors. Images obtained using the flash cytometer were analyzed automatically using the MetaMorph Neurite Outgrowth Application Module. Values are means ± S.D. (n = 6–8 wells).

Treatment
No. Surviving Cells/Well
Neurite Outgrowth/Cella
Processes/Cell
Branches/Cell
DMSO 137 ± 12 18.5 ± 2.4 1.17 ± 0.09 0.33 ± 0.07
Neurotrophic factors 428 ± 59* 39.4 ± 3.6* 2.18 ± 0.12* 1.20 ± 0.13*
TRO19622, 1 μM
190 ± 10*
28.5 ± 4.5*
1.48 ± 0.11*
0.66 ± 0.25*

  • ↵* Significantly different (p < 0.05) from control DMSO values using the Student's t test.

  • ↵ a Arbitrary unit (MetaMorph Neurite Outgrowth Application Module).