TABLE 2
Intracellular distribution of recombinant PDE4B5
COS1 cells were transfected to express PDE4B5, and its distribution between low-speed pellet (P1), high-speed pellet (P2), and high-speed supernatant (S2) cytosolic fractions was assessed. This distribution was followed by determining PDE4 activity both by using 1 μM cAMP as substrate and immunologically by using a PDE4B-specific antiserum to follow the distribution of the 58-kDa PDE4B5. Data show means ± S.D. for three separate experiments.