Prevention of hepatotoxicity and hepatic caspase-3-like activity by ATP-depleting carbohydrates in different models of cytokine-mediated liver injury
Carbohydrates were given at -30 min (LPS or GalN/LPS = 0) or at -60 min (ConA = 0). ALT and caspase-3 activity were measured at +8 h. Values are means ± S.E.M. (n).
Liver Injury Model | Carbohydrate Treatment | Plasma ALT Activity | DEVD Cleavage Activity |
|---|---|---|---|
| U/l | μU/mg | ||
| Control | Control | 25 ± 10 (3) | ≤25 |
| + Fructose | 55 ± 10 (3) | ≤25 | |
| + Tagatose | 25 ± 10 (3) | ≤25 | |
| + Mannose | 25 ± 10 (3) | ≤25 | |
| + Mannitol | 35 ± 15 (3) | ≤25 | |
| GalN/LPS | Control | 5730 ± 1140 (4) | 202 ± 48 |
| + Fructose | 650 ± 300 (6)** | 107 ± 48* | |
| + Tagatose | 90 ± 30 (6)** | 56 ± 53** | |
| + Mannose | 6280 ± 1610 (6) | 148 ± 45 | |
| + Mannitol | 7660 ± 670 (3) | 202 ± 17 | |
| LPS shock | Control | 2070 ± 180 (10) | ≤25 |
| + Fructose | 680 ± 220 (12)** | ≤25 | |
| + Tagatose | 100 ± 40 (3)** | ≤25 | |
| + Mannose | 1890 ± 220 (3) | ≤25 | |
| + Mannitol | 2370 ± 500 (3) | ≤25 | |
| ConA | Control | 3800 ± 540 (7) | ≤25 |
| + Fructose | 410 ± 90 (4)** | ≤25 | |
| + Tagatose | 200 ± 10 (3)** | ≤25 | |
| + Mannose | 2240 ± 380 (3) | ≤25 | |
| + Mannitol | 2090 ± 410 (3) | ≤25 | |
| GalN/SEB | Control | 5770 ± 1580 (4) | N.D. |
|
| + Fructose | 135 ± 60 (3)* | N.D. |