Compositions of solutions for recording various currents (in millimolars)
The pH of the bath solutions was 7.4, and the pH of the pipette solutions was 7.2, adjusted using appropriate hydroxide salts or HCl.
Current | Solution | Composition |
|---|---|---|
| IhERG, IKr-K | Bath: | 5 KCl, 130 NaCl, 1 MgCl2, 2 CaCl2, 10 glucose, 10 HEPES |
| Pipette: | 135 KCl, 10 EGTA, 1 MgCl2, 10 HEPES | |
| IKr-Cs | Bath: | 135 CsCl, 1 MgCl2, 10 glucose, 10 HEPES, 10 μM nifedipine |
| Pipette: | 135 CsCl, 10 EGTA, 5 MgATP, 10 HEPES | |
| INa | Bath: | 100 TEACl, 40 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 glucose, 10 HEPES |
| Pipette: | 135 CsCl, 10 EGTA, 5 MgATP, 10 HEPES | |
| IK1, Itoa | Bath: | 5.4 KCl, 130 NMDG, 1 MgCl2, 2 CaCl2, 10 glucose, 10 HEPES, 1 μM nifedipine |
| Pipette: | 135 KCl, 10 EGTA, 1 MgCl2, 5 MgATP, 10 HEPES | |
| AP | Bath: | 5 KCl, 130 NaCl, 1 MgCl2, 2 CaCl2, 10 glucose, 10 HEPES |
|
| Pipette: | 135 KCl, 10 EGTA, 1 MgCl2, 5 MgATP, 10 HEPES |
↵ a Ito was obtained using current subtraction method with Ito blocker 4-AP (10 mM).