TABLE 1

Cytotoxicity of ethonafide in human prostate cancer cell lines and normal prostate epithelial cells Cytotoxicity was determined by MTT. Area under the curve is represented in parentheses, and it was calculated as follows: time (hours) × IC50 (micromolar). IC50 of PReC cells was only determined for a 3-day exposure due to the slow and finite growth of these cells.


Time

Mean IC50
DU 145
PC-3
LNCaP
PReC
h μM
24 0.224 (5.4) 2.2 (52.8) 10 (240) N.D.
48 0.068 (3.3) 0.430 (20.6) 0.280 (13.4) N.D.
72 0.040 (2.9) 0.086 (6.2) 0.098 (7.1) 0.291 (20.9)
120
0.016 (1.9)
0.028 (3.4)
0.026 (3.1)
N.D.
  • N.D., not determined