Effect of CORM-3 on markers of HUVEC activation
Confluent T25 flasks of HUVECs were stimulated for 4 (E-selectin) or 24 h (ICAM-1, PECAM-1, and IL-8) with TNF-α in the presence or absence of CORM-3 or iCORM-3 (100 μM). Data for adhesion molecules are expressed as percentage of TNF-α-induced response, whereas for IL-8 are picograms per milliliter. In all cases, values are mean ± S.E.M. of three distinct experiments performed with different HUVEC batches (all nonsignificant compared to TNF-α alone).
Treatment | ICAM-1 Expression | PECAM-1 Expression | E-Selectin Expression | IL-8 |
|---|---|---|---|---|
| pg / ml | ||||
| Basal | 17 ± 2 | 107.5 ± 11.5 | 8 | 1.4 ± 0.1 |
| TNF-α | 100 | 100 | 100 | 17 ± 1.0 |
| + iCORM-3 | 85 ± 19 | 87.3 ± 8.4 | 93 ± 31.4 | 15.8 ± 1.6 |
| + CORM-3 [1 μM] | 92 ± 6.5 | 105.3 ± 9.9 | 123 | 18.9 ± 2.3 |
| + CORM-3 [10 μM] | 85.8 ± 21.9 | 97 ± 15.7 | 156 ± 7.6 | 19.9 ± 2.5 |
| + CORM-3 [100 μM] | 86 ± 6 | 109.3 ± 10.3 | 144 ± 46.9 | 16.2 ± 1.0 |