TABLE 2

Preproendothelin-1 gene expression, ECE activity, and ETB sites in RPAEC cultured under control conditions or in the presence of 10 pM exogenous AM given in combination with antagonists and inhibitors

For each group, n = 6.

CGRP(8-37)
H89
KT-5720
hAM(22-52)
l-NOARG
KT-5823
h
Preproendothelin-1 mRNA (% control)
6 109 ± 16 101 ± 10 89 ± 16 192 ± 22* 212 ± 20* 229 ± 36*
12 91 ± 9 99 ± 9 111 ± 9 299 ± 19* 280 ± 29* 275 ± 27*
ECE activity (ng ET-1/mg protein/h)
6 180 ± 17 193 ± 17 196 ± 20 289 ± 29* 249 ± 29* 261 ± 28*
12 160 ± 17 144 ± 16 166 ± 18 319 ± 40* 300 ± 40* 290 ± 30*
Maximal no. of ETB sites (fmol/mg protein)
6 178 ± 16 175 ± 17 180 ± 16 188 ± 16 173 ± 18 182 ± 18
12
175 ± 16
161 ± 12
179 ± 16
199 ± 26
156 ± 15
170 ± 19

  • ↵* P < 0.05 vs. untreated control (no AM, no drugs; see Table 1).