TABLE 1

Kinetic parameters of the uptake of organic anions by human kidney slices

Kinetic parameters were determined by nonlinear regression analysis using data shown in Fig. 5. The parameters within parentheses represent that of low-affinity components. Each value represents the mean ± S.D.


Substrate

Human Kidney Slices
Subject Nos.
Km
Vmax
Pdif
μM nmol / g kidney / 15 min
PAH 30 39.5 ± 1.0 62.9 ± 15.4 0.438 ± 0.048
31 31.1 ± 18.9 25.4 ± 14.9 0.412 ± 0.060
36 47.8 ± 26.4 50.1 ± 27.5 0.436 ± 0.079
2,4-D 20 3.94 ± 1.04 23.0 ± 5.5 1.65 ± 0.13
24 0.727 ± 0.449 7.03 ± 2.91 1.41 ± 0.23
30 4.85 ± 1.39 25.6 ± 6.2 1.09 ± 0.11
PCG 20 89.8 ± 44.0 103 ± 53 0.461 ± 0.096
24 42.9 ± 10.0 126 ± 27 0.439 ± 0.064
30 13.9 ± 4.6 22.9 ± 6.4 0.455 ± 0.041
DHEAS 3 3.57 ± 1.67 (1340 ± 260 19.2 ± 8.9 (5750 ± 760)
4 2.19 ± 0.91 (1270 ± 580) 20.9 ± 8.2 (3810 ± 1240)
5 3.91 ± 1.38 (1260 ± 490) 26.6 ± 10.3 (4800) ± 1340)
ES 37 9.18 ± 2.01 91.4 ± 17.4 2.01 ± 0.17

39
10.7 ± 2.2
148 ± 26
2.31 ± 0.21