Summary of binding assays conditions
Receptor | Species | Cell/Tissue (Amount/Assay) | Incubation Buffer | Incubation Time; Temperature; pH | Radioligand | Nonspecific | Reference |
|---|---|---|---|---|---|---|---|
| mM | nM | μM | |||||
| rD3 | Rat | Sf9 (10 μg) | 50 Tris-HCl, 5 MgCl2, 5 EDTA, 5 KCl, 1.5 CaCl2, 120 NaCl | 60 min, 27°C, 7.4 | [3H]Spiperone (0.4) | Haloperidol (10) | Catalog no. BSR-D3R, RBI Biosignal (Montreal, QC, Canada) |
| hD3a | Human | CHO (10 μg) | 50 Tris-HCl | 120 min, 37°C, 7.4 | [3H]Spiperone (0.7) | S-(–)sulpiride (25) | Sokoloff et al. (1990) |
| rD2 | Rat | Striatum (1.25 mg) | 50 Tris-HCl, 1 MgCl2, 5 EDTA, 5 KCl, 2 CaCl2, 120 NaCl | 15 min, 37°C, 7.4 | [3H]Spiperone (0.5) | (±)sulpiride (10) | Seeman (1983) |
| hD2La | Human | CHO (20 μg) | 50 Tris-HCl, 2 MgCl2, 5 EDTA, 5 KCl, 2 CaCl2, 120 NaCl | 120 min; 25°C; pH 7.4 | [3H]Spiperone (2.0) | Haloperidol (10) | Hayes et al. (1992) |
| rα-1 | Rat | Cerebral cortex (32 mg) | 50 Tris-HCl | 30, 23°C, 7.4 | [3H]Prazosin (0.5) | Phentolamine (10) | Greengrass and Bremner (1979) |
| r5-HT1A | Rat | Hippocampus (0.45 mg) | 50 Tris-HCl, 4 CaCl2, 0.1% ascorbic acid, 0.01 pargyline | 15 min, 37°C, 7.7 | [3H]8-OH-DPAT (2.0) | Serotonin (10) | Hall et al. (1985) |
| h5-HT1Aa | Human | CHO (8 μg) | 50 Tris-HCl | 60 min, 25°C, 7.4 | [3H]8-OH-DPAT (1.0) | Metergoline (10) | May et al. (2003) |
| Opiate (nonselective)a | Rat | Whole brain (7.5 mg) | 50 Tris-HCl | 40 min, 25°C, 7.7 | [3H]Naloxone (1.0) | Naloxone (1) | Pasternak et al. (1975) |
↵ a Assays were carried out by MDS Pharma Service; all other assays were done in-house