TABLE 2

Inhibition of PI-3K prevents IFNγ-induced E. coli internalization One million T84 epithelial cells were seeded into Petri dishes, and 3 to 4 days later, they were treated with 20 ng/ml IFNγ ± LY (20 μM) for 48 h. The medium was replaced with fresh antibiotic-free medium containing 10⁶ cfu of E. coli HB101. Sixteen hours later, extracellular bacteria were killed by gentamicin treatment, followed by epithelial lysis and enumeration of internalized bacteria (mean colony counts from four experiments, two to three epithelial preparations per condition per experiment).