Effect of Salvinorin A on DAMGO-mediated inhibition of forskolin-stimulated cAMP accumulation
Dose-response curves for DAMGO-mediated inhibition of forskolin-stimulated cAMP accumulation were generated as described under Materials and Methods. EC50 and Emax were determined using the program Prism. Each value is the mean ± S.E.M. (n = 3).
ED50 | EMAX | Apparent Ke | |
|---|---|---|---|
| nM ± S.D. | % maximal inhibition ± S.D. | ||
| DAMGO | 4.0 ± 0.7 | 85.6 ± 1.0 | |
| DAMGO + 10 μM Salvinorin A | 8.7 ± 2.4 | 81.8 ± 4.0 | 8.6 μM |
| DAMGO + 50 μM Salvinorin A | 11.6 ± 0.3** | 78.7 ± 1.9* | 27 μM |
| DAMGO + 10 nM naloxone | 18.9 ± 3.0** | 84.1 ± 2.0 | 2.7 nM |
↵* P < 0.05; ** P < 0.01.