[3H]Diprenorphine binding surfaces in hMOR-CHO cells
[3H]Diprenorphine binding surfaces were generated by displacing two concentrations of [3H]diprenorphine (0.08 and 0.47 nM) by nine concentrations of diprenorphine in the absence and presence of the indicated concentrations of either Salvinorin A or naloxone, generating 20 data points per experimental condition. The combined data of each condition (160 points for the Salvinorin A experiments, 120 points for the naloxone experiments) were fit to the one-site binding model using MLAB-PC for the best-fit estimates (± S.D.) of the Kd and Bmax values.
Test Drug | Bmax | Kd |
|---|---|---|
| nM | fmol / mg protein ± S.D. | nM ± S.D. |
| Salvinorin A (n = 4) | ||
| 0 | 288 ± 18 | 0.73 ± 0.04 |
| 10,000 | 186 ± 18* | 1.43 ± 0.11* |
| 40,000 | 190 ± 14* | 1.56 ± 0.12* |
| Naloxone | ||
| 0 | 515 ± 25 | 0.97 ± 0.05 |
| 10 | 445 ± 47 | 2.64 ± 0.25* |
↵* P < 0.001 (F-test).