Parameters of inhibition of forskolin-stimulated cAMP synthesis by carbachol in control and Gsα siRNA transfected CHO-M2 cells
Sigmoidal concentration-response curves with variable slope were fitted to the data shown in Fig. 2 (bottom). Difference indicates parameters of the cAMP synthesis concentration-response curve calculated as a difference between siRNA-treated and control cells. Values in parentheses indicate 95% confidence limits.
Treatment | Emax | EC50 | Hill Slope |
|---|---|---|---|
| % control | μM | ||
| Control | 58.5 (56.4-60.5) | 0.35 (0.31-0.40) | -1.67 (-1.97 to -1.36) |
| siRNA Gs | 48.7 (44.4-53.0) | 0.35 (0.25-0.48) | -1.72 (-2.61 to -0.85) |
| Difference | 139.3 (26.3-52.2) | 21.9 (3.4-141.3) | 0.53 (0.25-0.81) |