TABLE 2

Cellular localization of EYFP-hCAR in CAR-null mice

CAR–/– mice were tail vein-injected with 10 μ g of EYFP-hCAR and treated 2 and 5 h later by intraperitoneal injection of vehicle (DMSO, CTL), 50 mg/kg RIF, 100 mg/kg PB, 10 mg/kg CITCO, 20 mg/kg CMZ, 25 mg/kg EFV, or 50 mg/kg NVP at volumes of 0.1 ml per 20 g body weight. EYFP-hCAR expression was detected in mouse liver sections by confocal laser scanning microscope. For each treatment, over 150 hCAR-expressing cells from two mice livers (M1 and M2) were counted and classified as having cytosolic (C), nuclear (N), or mixed (C + N) hCAR localization. Numbers in parentheses represent the percentages of cells exhibiting the specified localization relative to the total number of counted cells. M1 = mouse 1, M2 = mouse 2.


Compounds

C

N

Mixed

Total
M1
M2
M1
M2
M1
M2
M1
M2
CTL 142 (88%) 152 (77%) 15 (9%) 35 (18%) 5 (3%) 10 (5%) 162 (100%) 197 (100%)
RIF (50 mg/kg) 155 (91%) 148 (82%) 10 (6%) 30 (16%) 5 (3%) 3 (2%) 170 (100%) 181 (100%)
PB (100 mg/kg) 59 (33%) 62 (27%) 113 (64%) 154 (67%) 6 (3%) 13 (6%) 178 (100%) 229 (100%)
CITCO (10 mg/kg) 67 (37%) 48 (30%) 84 (46%) 61 (40%) 32 (17%) 48 (30%) 183 (100%) 157 (100%)
CMZ (20 mg/kg) 21 (13%) 26 (14%) 118 (75%) 147 (81%) 18 (12%) 9 (5%) 157 (100%) 182 (100%)
EFV (25 mg/kg) 29 (19%) 41 (19%) 120 (78%) 163 (76%) 5 (3%) 11 (5%) 154 (100%) 215 (100%)
NVP (50 mg/kg)
46 (21%)
40 (21%)
129 (58%)
130 (67%)
47 (21%)
23 (12%)
222 (100%)
193 (100%)