Real-time PCR conditions
Gene | Initial Denaturation | Cycles | Denaturation | Annealing | Extension | Prolonged Extension |
|---|---|---|---|---|---|---|
| ANP | 94°C, 15 min | 34 | 94°C, 20 s | 59°C, 20 s | 72°C, 30 s | 72°C, 5 min |
| c-fos | 34 | 58°C, 25 s | 72°C, 30 s | |||
| 18S |
| 34 |
| 55°C, 20 s | 72°C, 30 s |
|