TABLE 1

Unbound liver concentrations and intrinsic clearance values for sulfate conjugates of acetaminophen, 4-methylumbelliferone, and harmol

Liver concentrations (C) and intrinsic clearance values (CI) were determined as described under Materials and Methods. Mean ± S.D., n = 3–4/group.

	C57BL/6	Abcc3^-/-	Abcc4^-/-
		Acetaminophen Sulfate
C_{liver unbound} (nM)	376 ± 59	321 ± 61	340 ± 53
Cl_basolateral (μl/min/g liver)	142 ± 24	117 ± 42	111 ± 20
Cl_bile (μl/min/g liver)	6 ± 1	10 ± 3^*	6 ± 1
		4-Methylumbelliferyl Sulfate
C_{liver unbound} (nM)	211 ± 55	308 ± 166	373 ± 91
Cl_basolateral (μl/min/g liver)	2036 ± 667	1012 ± 354^*	733 ± 235^*
Cl_bile (μl/min/g liver)	927 ± 332	762 ± 239	452 ± 179
		Harmol Sulfate
C_{liver unbound} (nM)	745 ± 125	537 ± 109	620 ± 51
Cl_basolateral (μl/min/g liver)	411 ± 131	296 ± 80	229 ± 10
Cl_bile (μl/min/g liver)^a	459 ± 42	670 ± 241	400 ± 41

↵* p < 0.05, knockout vs. wild type.
↵ a Statistical significance was assessed by ANOVA on ranks with Dunn's post hoc test.