Genes and primers: accession numbers of RefSeq mRNA sequences and sequences of primers used for real-time PCR analyses to evaluate the expression of transporter genes
Official Protein and Gene Symbol | Old Protein Name | Accession Number | Primer Sequence 5′ → 3′ | Localization | Product Length | Product Tm |
|---|---|---|---|---|---|---|
| bp | °C | |||||
| OATP1A2, (SLCO1A2) | OATP-A | NM_134431a | CCATTGGAACGGGAATAAACA (f) | 2136-2351 | 216 | 84.0 |
| TCTCTTCAGATTTCATACACCTCA (r) | ||||||
| OATP2B1, (SLCO2B1) | OATP-B | NM_007256 | GGACAACAGCCAGGTTTTCTACAC (f) | 1851-1958 | 108 | 88.5 |
| AGGAAGGGCACCACCAGATG (r) | ||||||
| OATP1B1, (SLCO1B1) | OATP-C | NM_006446 | TAAGGCTAACATCTTATTGGGAGTC (f) | 1250-1382 | 133 | 79.5 |
| CACAGCAGTAAAACATGAGAATTTGG (r) | ||||||
| OATP3A1, (SLCO3A1) | OATP-D | NM_013272 | AGCCCTGGACCCCTACTCG (f) | 1582-1716 | 135 | 86.5 |
| CCCGTGAGATTCGTGCTGTTG (r) | ||||||
| OATP4A1, (SCLO4A1) | OATP-E | NM_016354 | CACCAGTTGAAGGACAGCAG (f) | 1253-1341 | 89 | 86.5 |
| AGGAGCCAGATGGAGAGAGG (r) | ||||||
| OATP1B3, (SLCO1B3) | OATP-8 | NM_019844 | CCGTATTTTTTGGAAGGGTCTAC (f) | 1897-2034 | 138 | 79.5 |
| TTCTTTCATTGTCCGATGCC (r) | ||||||
| OAT2 (SLC22A7) | NLT | NM_153320a | TGCTAGTGTCCTCCGATATGA (f) | 1649-1748 | 100 | 84.5 |
| GAAGTGAACAGGTAGGCAGTG (r) | ||||||
| OAT3 (SLC22A8) | NM_004254 | CCCACAGTCATCAGGCAAACA (f) | 1448-1584 | 137 | 86.5 | |
| AGGGCGGTGATCCCGTAGA (r) | ||||||
| OCT1 (SLC22A1) | HOCT1 | NM_003057a | CGCCGAGAACCTTGGGAGAAA (f) | 1686-1795 | 110 | 84.5 |
| ACGACATCGCCGCAAAACATC (r) | ||||||
| NTCP (SLC10A1) | NTCP1 | NM_003049 | GGACATGAACCTCAGCATTG (f) | 424-586 | 163 | 85.5 |
| AATGAGAACCAGGACCAGTG (r) | ||||||
| PEPT-1 (SLC15A1) | NM_005073 | CACCTCCTTGAAGAAGATGGCA (f) | 1121-1225 | 105 | 86.0 | |
| GGGAAGACTGGAAGAGTTTTATCG (r) | ||||||
| PEPT-2 (SLC15A2) | NM_021082 | TTTGTCTCTCTACACTGAGCATTC (f) | 1453-1563 | 111 | 81.5 | |
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| CTGCTTTCTGTATCCTTTACCATC (r) |
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f, forward; r, reverse.
↵ a More than one transcript variant is available and the primers for these genes span common area for all transcript variants.