PT  - JOURNAL ARTICLE
AU  - Min Zhang
AU  - Feifei Luo
AU  - Yufei Zhang
AU  - Luman Wang
AU  - Wei Lin
AU  - Mengxuan Yang
AU  - Dali Hu
AU  - Xiaofeng Wu
AU  - Yiwei Chu
TI  - <em>Pseudomonas aeruginosa</em> Mannose-Sensitive Hemagglutinin Promotes T-Cell Response via Toll-Like Receptor 4–Mediated Dendritic Cells to Slow Tumor Progression in Mice
AID  - 10.1124/jpet.113.212316
DP  - 2014 May 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 279--287
VI  - 349
IP  - 2
4099  - http://jpet.aspetjournals.org/content/349/2/279.short
4100  - http://jpet.aspetjournals.org/content/349/2/279.full
SO  - J Pharmacol Exp Ther2014 May 01; 349
AB  - Pseudomonas aeruginosa–mannose-sensitive hemagglutinin (PA-MSHA) as a drug may kill tumor cells and has been used clinically. However, the antitumor immune response of PA-MSHA is not completely understood. In this study, we found that treating Lewis lung carcinoma (3LL)-bearing mice with PA-MSHA plus 3LL antigen led to slower tumor progression and longer survival. After PA-MSHA treatment, T-cell number and dendritic cell maturation were both increased significantly at the tumor site. In addition, PA-MSHA in vitro stimulation resulted in the maturation of bone marrow–derived dendritic cells (BMDCs) from naive mice, showing higher costimulatory molecule expression, more cytokine secretion, lower endocytic activity, and stronger capacity to enhance T-cell activation. Toll-like receptor (TLR)4 but not TLR2 was required in the maturation process. More importantly, PA-MSHA–induced DCs were essential for PA-MSHA to enhance activation, expansion, and interferon (IFN)-γ secretion of TLR4-mediated T cells, which play a role in the antitumor effect of PA-MSHA. Thus, this study reveals PA-MSHA as a novel TLR4 agonist that elicits antitumor immune response to slow tumor progression.