PT - JOURNAL ARTICLE AU - Grinnell, Steven G. AU - Majumdar, Susruta AU - Narayan, Ankita AU - Le Rouzic, Valerie AU - Ansonoff, Michael AU - Pintar, John E. AU - Pasternak, Gavril W. TI - Pharmacologic Characterization in the Rat of a Potent Analgesic Lacking Respiratory Depression, IBNtxA AID - 10.1124/jpet.114.213199 DP - 2014 Sep 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 710--718 VI - 350 IP - 3 4099 - http://jpet.aspetjournals.org/content/350/3/710.short 4100 - http://jpet.aspetjournals.org/content/350/3/710.full SO - J Pharmacol Exp Ther2014 Sep 01; 350 AB - IBNtxA (3′-iodobenzoyl-6β-naltrexamide) is a potent analgesic in mice lacking many traditional opioid side effects. In mice, it displays no respiratory depression, does not produce physical dependence with chronic administration, and shows no cross-tolerance to morphine. It has limited effects on gastrointestinal transit and shows no reward behavior. Biochemical studies indicate its actions are mediated through a set of μ-opioid receptor clone MOR-1 splice variants associated with exon 11 that lack exon 1 and contain only six transmembrane domains. Like the mouse and human, rats express exon 11–associated splice variants that also contain only six transmembrane domains, raising the question of whether IBNtxA would have a similar pharmacologic profile in rats. When given systemically, IBNtxA is a potent analgesic in rats, with an ED50 value of 0.89 mg/kg s.c., approximately 4-fold more potent than morphine. It shows no analgesic cross-tolerance in morphine-pelleted rats. IBNtxA displays no respiratory depression as measured by blood oxygen saturation. In contrast, oximetry shows that an equianalgesic dose of morphine lowers blood oxygen saturation values by 30%. IBNtxA binding is present in a number of brain regions, with the thalamus standing out with very high levels and the cerebellum with low levels. As in mice, IBNtxA is a potent analgesic in rats with a favorable pharmacologic profile and reduced side effects.