PT  - JOURNAL ARTICLE
AU  - JEROME A. SCHACK
AU  - SAMUEL H. WAXLER
TI  - AN ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF THEOPHYLLINE AND THEOBROMINE IN BLOOD AND TISSUES
DP  - 1949 Nov 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 283--291
VI  - 97
IP  - 3
4099  - http://jpet.aspetjournals.org/content/97/3/283.short
4100  - http://jpet.aspetjournals.org/content/97/3/283.full
SO  - J Pharmacol Exp Ther1949 Nov 01; 97
AB  - 1. A simple, rapid and accurate ultraviolet spectrophotometric method for the determination of theophylline and theobromine in biological fluids has been developed. 2. The method offers satisfactory precision and a sensitivity of 1.0 microgm. per cc. 3. Application of the method to the tissue of rabbits and mice has been shown. 4. Theophylline has been demonstrated to be restricted to the plasma of the circulating blood. 5. Theophylline has been demonstrated to be very loosely bound to the plasma proteins. 6. The tissue level of theophylline following intravenous administration has been found to parallel the blood level in its rate of fall. 1949 by The American Society for Pharmacology and Experimental Therapeutics