RT Journal Article
SR Electronic
T1 THE RELATIONSHIP BETWEEN CHOLINESTERASE INHIBITION AND THE PHARMACOLOGICAL ACTION OF DI-ISOPROPYL FLUOROPHOSPHATE (DFP)
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 421
OP 434
VO 87
IS 4
A1 GEORGE B. KOELLEL
A1 ALFRED GILMAN
A1 BERTHA D. BINZER
YR 1946
UL http://jpet.aspetjournals.org/content/87/4/421.abstract
AB 1. Rats, dogs and monkeys appeared to show no physiological abnormality when the cholinesterase of the blood and tissues was reduced to a low percentage of its normal activity by di-isopropyl fluorophosphate. The signs associated with further reduction are described. 2. It was found that determinations of serum and red cell cholinesterase inhibition following the administration of di-isopropyl fluorophosphate do not provide accurate estimates of the activity of the enzyme in the tissues, excepting possibly during the period immediately following a single dose. Such measurements can be used advantageously to follow rates of absorption. 3. No deterioration was detected in solutions of di-isopropyl fluorophosphate in peanut oil following autoclaving for one hour or storage at room temperature for one year. 4. The concentration of a peanut oil solution of di-isopropyl fluorophosphate had little effect on its rate of absorption from an intramuscular site in dogs. 5. The effective intramuscular:oral dosage ratio for di-isopropyl fluorophosphate in peanut oil was found to be approximately 1:4 in monkeys and humans.