PT - JOURNAL ARTICLE AU - R. G. TURNER AU - E. R. LOEW TI - BLOOD ALCOHOL AND ITS RELATION TO INTOXICATION DP - 1932 Mar 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 305--324 VI - 44 IP - 3 4099 - http://jpet.aspetjournals.org/content/44/3/305.short 4100 - http://jpet.aspetjournals.org/content/44/3/305.full SO - J Pharmacol Exp Ther1932 Mar 01; 44 AB - 1. A method has been described for the detection of blood alcohol based on the Naville modification of the Nicloux method, from which it differs in that the proteins are precipitated with the ordinary Folin-Wu reagents and the distillation is carried out with a modified ice condenser. 2. The average per cent error found by the Nicloux method in estimating alcohol in aqueous solutions is 4.31. The range varies from 0.8 to 13.0 per cent, the greater error resulting in the estimation of amounts of alcohol below 10.5 mgm. 3. When applied to blood distillates the method gives an average per cent error of 5.09 with a range of 0.3 to 15.0. The greater error is encountered in blood samples containing less than 10.5 mgm. of alcohol. 4. Thirteen blood alcohol absorption curves on dogs are discussed. The determinations were made after giving 10, 20, 25 and 40 per cent alcohol solutions during fasting, after eating and during intervals of feeding. The alcohol content of the blood was determined by Naville's modification of the Nicloux method. 5. Intoxication in the dog is definite, if within one-half to two hours after ingestion the alcohol content of the blood measures 0.25 per cent; at a blood alcohol concentration of 0.3 per cent, intoxication is marked and a concentration of 0.4 to 0.5 per cent is accompanied by alcoholic stupor. 6. The alcohol content of the blood remains at a higher level for a more prolonged period after ingestion of strong solutions of alcohol (40 per cent) than occurs after consumption of weak solutions (10 per cent). 7. The rate of destruction of alcohol in the blood after ingestion of weak or strong solutions is the same for animals not accustomed to alcohol. The rate increased 60 per cent in an animal accustomed to alcohol. 8. When alcohol is consumed during a meal or following a meal intoxication is not as marked or the rise in blood alcohol as great as when the same quantity is given during fasting. 9. The method described for estimation of alcohol in blood is believed to be reliable and sufficiently accurate to diagnose intoxication.