PT - JOURNAL ARTICLE AU - Edwin C. Y. Chow AU - Matthew R. Durk AU - Carolyn L. Cummins AU - K. Sandy Pang TI - 1α,25-Dihydroxyvitamin D<sub>3</sub> Up-Regulates P-Glycoprotein via the Vitamin D Receptor and Not Farnesoid X Receptor in Both <em>fxr</em>(−/−) and <em>fxr</em>(+/+) Mice and Increased Renal and Brain Efflux of Digoxin in Mice In Vivo AID - 10.1124/jpet.111.179101 DP - 2011 Jun 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 846--859 VI - 337 IP - 3 4099 - http://jpet.aspetjournals.org/content/337/3/846.short 4100 - http://jpet.aspetjournals.org/content/337/3/846.full SO - J Pharmacol Exp Ther2011 Jun 01; 337 AB - Secondary farnesoid X receptor (FXR) effects, in addition to vitamin D receptor (VDR) effects, were observed in the rat liver after treatment with 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3], the natural ligand of VDR, caused by increased bile acid absorption as a consequence of apical sodium-dependent bile acid transporter induction. To investigate whether the increased multidrug resistance protein 1 (Mdr1)/P-glycoprotein (P-gp) expression in the rat liver and kidney was caused by the VDR and not the FXR, we examined changes in Mdr1/P-gp expression in fxr(+/+) and fxr(−/−) mice after intraperitoneal dosing of vehicle versus 1,25(OH)2D3 (0 or 2.5 μg/kg every other day for 8 days). Renal and brain levels of Mdr1 mRNA and P-gp protein were significantly increased in both fxr(+/+) and fxr(−/−) mice treated with 1,25(OH)2D3, confirming that Mdr1/P-gp induction occurred independently of the FXR. Increased P-gp function was evident in 1,25(OH)2D3-treated fxr(+/+) mice given intravenous bolus doses of the P-gp probe, [3H]digoxin (0.1 mg/kg). Decreased blood (24%) and brain (29%) exposure, estimated as reduced areas under the curve, caused by increased renal (74%) and total body (34%) clearances of digoxin, were observed in treated mice. These events were predicted by physiologically based pharmacokinetic modeling that showed increased renal secretory intrinsic clearance (3.45-fold) and brain efflux intrinsic clearance (1.47-fold) in the 1,25(OH)2D3-treated mouse, trends that correlated well with increases in P-gp protein expression in tissues. The clearance changes were less apparent because of the high degree of renal reabsorption of digoxin. The observations suggest an important role of the VDR in the regulation of P-gp in the renal and brain disposition of P-gp substrates.