@article {Wang847, author = {Xiaoli Wang and Yi Guo and Shu Yang and Caihong Wang and Xuping Fu and Jinling Wang and Yumin Mao and Junsong Zhang and Yao Li}, title = {Cellular and Molecular Mechanisms of Photodynamic Hypericin Therapy for Nasopharyngeal Carcinoma Cells}, volume = {334}, number = {3}, pages = {847--853}, year = {2010}, doi = {10.1124/jpet.110.168856}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Hypericin-mediated photodynamic therapy (HY-PDT) has become a potential treatment for tumors and nonmalignant disorders. Some studies reported that HY-PDT could lead to apoptosis in some carcinoma cells. However, the molecular mechanism of HY-PDT remains unknown. In this study, we evaluated the molecular mechanisms of hypericin associated with light-emitting diode irradiation on the poorly differentiated human nasopharyngeal carcinoma cell line CNE-2 in vitro. To comprehensively understand the effects of HY-PDT on CNE-2 cells, we detected cell viability, cell cycle, apoptosis, intracellular glutathione content, and intracellular caspase (caspase-9, caspase-3, and caspase-8) activity. Furthermore, we performed genome-wide expression analysis via microarrays at different time points in response to HY-PDT, and we found that differentially expressed genes were highly enriched in the pathways related to reactive oxygen species generation, mitochondrial activity, DNA replication and repair, cell cycle/proliferation, and apoptosis. These results were consistent with our cytology test results and demonstrated that caspase-dependent apoptosis occurred after HY-PDT. Taken together, both cellular and molecular data revealed that HY-PDT could inhibit the growth of CNE-2 cells and induce their apoptosis.}, issn = {0022-3565}, URL = {https://jpet.aspetjournals.org/content/334/3/847}, eprint = {https://jpet.aspetjournals.org/content/334/3/847.full.pdf}, journal = {Journal of Pharmacology and Experimental Therapeutics} }