PT  - JOURNAL ARTICLE
AU  - Haodan Yuan
AU  - Bo Feng
AU  - Ying Yu
AU  - Jonathan Chupka
AU  - Jenny Y. Zheng
AU  - Timothy G. Heath
AU  - Brian R. Bond
TI  - Renal Organic Anion Transporter-Mediated Drug-Drug Interaction between Gemcabene and Quinapril
AID  - 10.1124/jpet.108.149476
DP  - 2009 Jul 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 191--197
VI  - 330
IP  - 1
4099  - http://jpet.aspetjournals.org/content/330/1/191.short
4100  - http://jpet.aspetjournals.org/content/330/1/191.full
SO  - J Pharmacol Exp Ther2009 Jul 01; 330
AB  - In humans and rats, a synergistic blood pressure reduction was observed when the fibrate gemcabene (CI-1027) was coadministered with the angiotensin-converting enzyme inhibitor quinapril. In a quinapril (3 mg/kg) pharmacokinetic rat study, there was a 40% decrease in urinary excretion and a 53% increase in plasma area under the curve from 0 to 24 h of the active metabolite quinaprilat when coadministered with gemcabene (30 mg/kg). This observation revealed a possible transporter-mediated drug-drug interaction (DDI) between gemcabene and quinapril. This led to a series of studies investigating the underlying clearance mechanisms associated with these compounds intended to elucidate renal transporter interactions between quinapril and gemcabene. In vitro transporter studies using human embryonic kidney 293 cells transfected with human or rat organic anion transporter 3 (hOAT3, rOat3) revealed that quinaprilat is a substrate in both species, with a Km value of 13.4 μM for hOAT3. Subsequent studies discovered that gemcabene inhibited quinaprilat uptake by hOAT3 and rOat3 at IC50 values of 35 and 48 μM, respectively. Moreover, gemcabene acylglucuronide, the major metabolite of gemcabene glucuronidation, also inhibited hOAT3- and rOat3-mediated uptake of quinaprilat at IC50 values of 197 and 133 μM, respectively. High plasma concentrations of gemcabene (>100 μM) achieved in humans and rats upon oral dosing corroborate with gemcabene inhibition of renal OAT3-mediated secretion of quinaprilat in vitro. This investigation established that a DDI between gemcabene and quinapril involving inhibition of renal transporters and subsequent elevation in plasma concentrations of quinaprilat is responsible for the apparent synergistic blood pressure reduction observed with these compounds.