RT Journal Article SR Electronic T1 7-Epiclusianone, a Tetraprenylated Benzophenone, Relaxes Airway Smooth Muscle through Activation of the Nitric Oxide-cGMP Pathway JF Journal of Pharmacology and Experimental Therapeutics JO J Pharmacol Exp Ther FD American Society for Pharmacology and Experimental Therapeutics SP 206 OP 214 DO 10.1124/jpet.108.138032 VO 327 IS 1 A1 Luciana Pontes Coelho A1 Magda Fráguas Serra A1 Ana Lúcia de Aguiar Pires A1 Renato Sérgio Balão Cordeiro A1 Patrícia Machado Rodrigues e Silva A1 Marcelo Henrique dos Santos A1 Marco Aurélio Martins YR 2008 UL http://jpet.aspetjournals.org/content/327/1/206.abstract AB This study was undertaken to investigate the putative mechanism(s) underlying the antispasmodic effect of 7-epiclusianone, a naturally occurring compound isolated from the plant Garcinia brasiliensis. Guinea pig tracheal rings were mounted in tissue baths filled with Krebs' solution, and the contractile response to distinct stimuli was measured in the presence or absence of 7-epiclusianone. We also tested the effect of 7-epiclusianone on methacholine-evoked airways obstruction in BALB/c mice using barometric plethysmography. 7-Epiclusianone (10 μM) inhibited epithelium-intact tracheal ring contraction induced by allergen, histamine, 5-hydroxytryptamine, or carbachol challenge. The relaxation effect was abrogated by epithelium removal, the presence of nitric-oxide synthase inhibitor Nω-nitro-l-arginine methyl ester (l-NAME) (100 μM), or soluble guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) (10 μM). 7-Epiclusianone (1–100 μM) induced a dose-dependent increase in the intracellular cGMP levels of cultured tracheal rings. The relaxation effect of 7-epiclusianone was also inhibited by K+ channel blockers tetraethylammonium (10 μM), glibenclamide (1 μM), or apamin (1 μM), but not by 9-(tetrahydro-2′-furyl)adenine (SQ22,536) (100 μM), an adenylate cyclase inhibitor. In epithelium-intact tracheal rings, 7-epiclusianone also inhibited Ca2+-induced contractions in K+ (60 mM)-depolarized preparations, but it seemed ineffective in assays in which epithelium-denuded tracheal ring preparations were used. Oral administration of 7-epiclusinone (25–100 mg/kg) dose-dependently inhibited airway obstruction triggered by aerosolized methacholine (6–25 mg/ml), in a mechanism sensitive to l-NAME (20 mg/kg). In conclusion, the relaxation effect of 7-epiclusinone seems to be mediated by epithelium-, nitric oxide-, and cGMP-dependent mechanisms. Furthermore, oral administration of 7-epiclusianone reduces episodes of bronchial obstruction, warranting further research on this compound regarding a putative application in asthma therapy. The American Society for Pharmacology and Experimental Therapeutics