TY - JOUR T1 - Angiotensin II Type 1 Receptor-Dependent Nuclear Factor-κB Activation-Mediated Proinflammatory Actions in a Rat Model of Obstructive Acute Pancreatitis JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 10 LP - 18 DO - 10.1124/jpet.107.124891 VL - 323 IS - 1 AU - Yuk Cheung Chan AU - Po Sing Leung Y1 - 2007/10/01 UR - http://jpet.aspetjournals.org/content/323/1/10.abstract N2 - Angiotensin II is a key mediator of inflammation, and nuclear factor-κB (NF-κB) plays a critical role in various inflammatory diseases, including acute pancreatitis (AP). This study sought to elucidate the mechanism mediating angiotensin II involvement in angiotensin II type 1 (AT1) receptor-mediated NF-κB activation, and ultimately in proinflammatory actions of AP pathogenesis. A rat model of obstructive pancreatitis was induced by ligation of the common biliopancreatic duct. Pancreatic injury was determined by assessing pancreatic histology, myeloperoxidase activity, and serum interleukin-6. Protein levels of pancreatic angiotensinogen and AT1 receptor as well as NF-κB inhibitory subunits (IκBα and IκBβ) and phospho-NF-κB p65, κB-related proteins (intercellular adhesion molecule-1, cyclooxygenas-2, and interleukin-1), and NADPH oxidase isoforms p67 and p22 were examined by Western blot. Nuclear κB binding activity and degree of oxidative stress were determined by electrophoretic mobility shift assay and glutathione/nitrotyrosine examination, respectively. The effects of losartan, an AT1 receptor antagonist, on NF-κB-mediated proinflammatory actions were also assessed. Induction of AP was associated with a time-dependent increase in pancreatic angiotensinogen levels. AT1 receptor blockade with losartan improved the pancreatic histological damage, myeloperoxidase activity, and serum interleukin-6. Losartan treatment also reduced AP-associated depletion of IκBβ and elevation of phospho-NF-κB p65 protein expression as well as the enhanced nuclear κB binding activity and elevated levels of κB-related proteins. In addition, losartan treatment suppressed pancreatic glutathione and nitrotyrosine levels, which were consistent with decreased NADPH oxidase expression. These data provide substantial evidence that angiotensin II is involved in AT1 receptor-mediated NADPH oxidase-dependent NF-κB activation; thus, it might ultimately promote proinflammatory actions during AP pathogenesis. The American Society for Pharmacology and Experimental Therapeutics ER -