RT Journal Article
SR Electronic
T1 THE ELIMINATION OF NICOTIN IN THE MILK
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 1
OP 6
VO 32
IS 1
A1 ROBERT A. HATCHER
A1 HILDA CROSBY
YR 1927
UL http://jpet.aspetjournals.org/content/32/1/1.abstract
AB 1. Nicotin can be detected in an extract after the addition of 1 mgm. to 500,000 parts of milk. 2. The injection of a total of 0.005 mgm. of nicotin into the lymph sac of a frog weighing 20 grams (0.25 mgm. per kilogram) caused a great increase in respiration for a short time; a dose corresponding to 0.4 mgm. per kilogram caused, in addition, fibrillation in the perineum and subsequent depression in another frog; a dose corresponding to 0.5 mgm. of nicotin per kilogram caused these symptoms somewhat intensified, together with fibrillation of various muscles. 3. Immediately after making a purified extract alkaline the characteristic odor of nicotin can be detected in amounts less than those required to produce perceptible effects in a frog weighing 20 grams. 4. Large doses of nicotin suppress the secretion of milk in the cat and the cow for longer periods than those required for the elimination of the greater part of the poison from the body. No attempt was made to determine the mechanism by which the secretion is inhibited, but it is probable that the nerve end apparatus in the gland is involved. 5. Six doses (or more), corresponding to 0.5 mgm. each of nicotin per kilogram, can be injected intramuscularly in the cat at intervals of half an hour without causing lasting injury; the injection of four doses of 1.25 mgm. each in a cow in a period of about three hours and a half was apparently nearly fatal.