PT - JOURNAL ARTICLE AU - Cathérine Gebhard AU - Fotios Petroktistis AU - Hua Zhang AU - Daniel Kammerer AU - Christoph Köhle AU - Karin Klingel AU - Margitta Albinus AU - Christoph H. Gleiter AU - Hartmut Osswald AU - Almut Grenz TI - Role of Renal Nerves and Salt Intake on Erythropoietin Secretion in Rats following Carbon Monoxide Exposure AID - 10.1124/jpet.106.105973 DP - 2006 Oct 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 111--116 VI - 319 IP - 1 4099 - http://jpet.aspetjournals.org/content/319/1/111.short 4100 - http://jpet.aspetjournals.org/content/319/1/111.full SO - J Pharmacol Exp Ther2006 Oct 01; 319 AB - Because the data from the literature contain conflicting results regarding the role of renal nerves and angiotensin II in hypoxia-induced erythropoietin (EPO) secretion, we evaluated the effect of renal nerves and salt intake in rats on EPO secretion stimulated by carbon monoxide (CO). Serum levels and renal mRNA content of EPO were similarly elevated by exposure to different CO concentrations in a dose-dependent manner in rats with bilateral renal denervation (DNX) and in sham-denervated controls (INN). However, at 600 ppm CO, serum concentrations and mRNA of EPO were significantly higher in DNX compared with INN rats (p < 0.05). This increase of EPO secretion in DNX rats could be blocked by administration of neuropeptide Y (NPY) (p < 0.05), whereas the NPY receptor antagonist did not enhance EPO secretion in INN rats after CO exposure. Agonists and antagonists of β-adrenergic receptors had no effect on EPO secretion. High-salt (HS) diet reduced EPO secretory response at 600 ppm CO by 55% compared with INN rats on normal salt diet (p < 0.01). In addition, DNX increased EPO secretion in rats on low-salt and HS diet, whereas plasma renin activity did not correlate with EPO levels under these experimental conditions. In summary, our data suggest that renal nerves contribute to the half-maximal EPO secretory response to CO exposure, possibly via NPY receptors. The American Society for Pharmacology and Experimental Therapeutics