RT Journal Article
SR Electronic
T1 Clovamide-Type Phenylpropenoic Acid Amides, <em>N</em>-Coumaroyldopamine and <em>N</em>-Caffeoyldopamine, Inhibit Platelet-Leukocyte Interactions via Suppressing P-Selectin Expression
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 813
OP 819
DO 10.1124/jpet.105.097337
VO 317
IS 2
A1 Jae B. Park
A1 Norberta Schoene
YR 2006
UL http://jpet.aspetjournals.org/content/317/2/813.abstract
AB N-Coumaroyldopamine and N-caffeoyldopamine are clovamide-type phenylpropenoic acid amides found in Theobroma cacao. In this article, N-coumaroyldopamine and N-caffeoyldopamine were investigated to determine their effects on P-selectin expression and platelet-leukocyte interactions in vitro and in vivo models. At the concentration of 0.05 μM, they were able to inhibit P-selectin expression on the platelets by 33 (P &lt; 0.011) and 30% (P &lt; 0.012), respectively. The inhibition was partially blocked by β2-adrenoceptor antagonists, suggesting that β2 receptors are probably engaged in the inhibition. N-Caffeoyldopamine and N-coumaroyldopamine could also suppress platelet-leukocyte interactions in blood samples by 36 (P &lt; 0.013) and 32% (P &lt; 0.011), respectively, at the same concentration (0.05 μM). In an animal study, mice administrated orally with N-caffeoyldopamine (50 and 100 μg/35 g of body weight) also showed great reduction in the P-selectin expression and platelet-leukocyte interactions by 31 to 45% (P &lt; 0.011) and 34 to 43% (P &lt; 0.014), respectively. These data suggest that the clovamide-type phenylpropenoic acid amides are able to suppress platelet-leukocyte interactions via inhibiting P-selectin expression. The American Society for Pharmacology and Experimental Therapeutics