PT  - JOURNAL ARTICLE
AU  - Brian E. Reese
AU  - Collin Davidson
AU  - Melvin L. Billingsley
AU  - Jong Yun
TI  - Protein Kinase Cϵ Regulates Tumor Necrosis Factor-α-Induced Stannin Gene Expression
AID  - 10.1124/jpet.105.084236
DP  - 2005 Jul 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 61--69
VI  - 314
IP  - 1
4099  - http://jpet.aspetjournals.org/content/314/1/61.short
4100  - http://jpet.aspetjournals.org/content/314/1/61.full
SO  - J Pharmacol Exp Ther2005 Jul 01; 314
AB  - Stannin (Snn) is a highly conserved vertebrate protein that has been closely linked to trimethyltin (TMT) toxicity. We have previously demonstrated that Snn is required for TMT-induced cell death. Others have shown that TMT exposure results in tumor necrosis factor-α (TNFα) production and that TNFα treatment induces Snn gene expression in human umbilical vein endothelial cells (HUVECs). In this study, we investigated a signaling mechanism by which Snn gene expression is regulated by TMT and demonstrated that TNFα stimulates Snn gene expression in a protein kinase Cϵ-dependent manner in HUVECs in response to TMT exposure. Supporting this, we show that TMT-induced toxicity is significantly blocked by pretreatment with an anti-TNFα antibody in HUVECs. Using a quantitative real-time polymerase chain reaction assay, we also show that the level of Snn gene expression is significantly increased in HUVECs in response to either TMT or TNFα treatment. This TNFα-induced Snn gene expression is blocked when HUVECs were pretreated with bisindolylmaleimide I, an inhibitor of protein kinase C (PKC). In contrast, when HUVECs were treated with phorbol 12-myristate 13-acetate, a PKC activator, we observed a significant increase in Snn gene expression. Using isotype-specific siRNA against PKC, we further show that knockdown of PKCϵ, but not PKCδ or PKCζ, significantly blocked TNFα-induced Snn gene expression. Together, these results indicate that TNFα-induced, PKCϵ-dependent Snn expression may be a critical factor in TMT-induced cytotoxicity. The American Society for Pharmacology and Experimental Therapeutics