PT  - JOURNAL ARTICLE
AU  - Carlos Hermenegildo
AU  - Pilar J. Oviedo
AU  - Miguel Angel García-Pérez
AU  - Juan J. Tarín
AU  - Antonio Cano
TI  - Effects of Phytoestrogens Genistein and Daidzein on Prostacyclin Production by Human Endothelial Cells
AID  - 10.1124/jpet.105.090456
DP  - 2005 Nov 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 722--728
VI  - 315
IP  - 2
4099  - http://jpet.aspetjournals.org/content/315/2/722.short
4100  - http://jpet.aspetjournals.org/content/315/2/722.full
SO  - J Pharmacol Exp Ther2005 Nov 01; 315
AB  - The molecular mechanisms of the vascular effects of phytoestrogens are poorly studied. Prostacyclin is a potent vasodilator synthesized by two isoforms of cyclooxygenase (COX) in endothelium. This study examine the effects of two phytoestrogens, the isoflavones genistein and daidzein, on prostacyclin production by cultured human umbilical vein endothelial cells (HUVECs) and the possible role of not only estrogen receptors but also both COX isoforms. The two phytoestrogens significantly increased prostacyclin release in a time- and dose-dependent (0.01-1 μM) manner, being higher than control after 24 h. Selective inhibitors of COX-1, SC-560 [5-(4-chlorophenyl)-1-(4-methoxypjenyl)-3-(trifluoromethyl)-1H-pyrazole], and COX-2, NS-398 (N-[2-(cyclohexyloxy)-4 nitrophenyl]-methanesulfonamide), were used to investigate the relative contribution of each enzyme. Both inhibitors decreased basal production of prostacyclin, but only COX-2 inhibition completely abolished the isoflavone-stimulated prostacyclin production. Phytoestrogens also increased COX-2 mRNA expression and protein content without affecting COX-1 levels. All these effects were mediated through estrogen receptor activation since treatment of cells with the estrogen receptor antagonist ICI 182780 [7α-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]-estra-1,3,5(10)-triene-3,17β diol] completely abolished the isoflavone-induced increase in prostacyclin production, COX-2 mRNA expression, and COX-2 protein content. The results clearly support the hypothesis that genistein and daidzein increased HUVEC prostacyclin production through estrogen receptor-dependent mechanism, which involved the enhancement of COX-2 protein and activity. The American Society for Pharmacology and Experimental Therapeutics