PT - JOURNAL ARTICLE AU - Thai, Hoang AU - Wollmuth, Jason AU - Goldman, Steven AU - Gaballa, Mohamed TI - Angiotensin Subtype 1 Receptor (AT<sub>1</sub>) Blockade Improves Vasorelaxation in Heart Failure by Up-Regulation of Endothelial Nitric-Oxide Synthase via Activation of the AT<sub>2</sub> Receptor AID - 10.1124/jpet.103.054916 DP - 2003 Dec 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 1171--1178 VI - 307 IP - 3 4099 - http://jpet.aspetjournals.org/content/307/3/1171.short 4100 - http://jpet.aspetjournals.org/content/307/3/1171.full SO - J Pharmacol Exp Ther2003 Dec 01; 307 AB - To determine whether angiotensin receptor blockade decreases vascular tone in heart failure by improving endothelial-dependent vasorelaxation and increasing nitric oxide (NO) bioavailability, we treated infarcted adult male Sprague-Dawley rats with candesartan for 7 days or 8 weeks (10 mg/kg/day in drinking water). Candesartan, at both time points, lowered left ventricular (LV) systolic pressure (P &lt; 0.05) (122 ± 22 versus 74 ± 16 and 73 ± 10 mm Hg) and LV dP/dt (5914 ± 1294 versus 2857 ± 1672 versus 3175 ± 769 mm Hg/s), but lowered LV end-diastolic pressure only at 8 weeks (16.9 ± 9.7 versus 11.2 ± 5.7 versus 6.9 ± 5.3 mm Hg). The vasorelaxation response to acetylcholine (ACh) in thoracic aortic segments was decreased with infarction (P &lt; 0.05), remained unchanged with 1 week of candesartan, but increased 84 and 86% at 10–4 and 10–5 M ACh (P &lt; 0.05) at 8 weeks. The enhanced candesartan-induced vasorelaxation at 8 weeks was abolished with NG-nitro-l-arginine methyl ester (200 μM). In bovine pulmonary endothelial cells, 20 μM candesartan increased endothelial nitric-oxide synthase (eNOS) protein levels (P &lt; 0.05) (28.9 ± 2.6 versus 16.1 ± 3.7 intensity units/μg of protein); the increased eNOS was abolished by a specific angiotensin subtype 2 (AT2) receptor antagonist, PD 123319. These data suggest that AT1 receptor blockade enhances vasorelaxation in heart failure by increasing NO bioavailability, in part via an AT2 receptor-mediated up-regulation of eNOS protein. The American Society for Pharmacology and Experimental Therapeutics