RT Journal Article
SR Electronic
T1 Phytoestrogen Cimicifugoside-Mediated Inhibition of Catecholamine Secretion by Blocking Nicotinic Acetylcholine Receptor in Bovine Adrenal Chromaffin Cells
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 641
OP 649
DO 10.1124/jpet.103.062331
VO 309
IS 2
A1 Woo, Kyung-Chul
A1 Park, Yong-Soo
A1 Jun, Dong-Jae
A1 Lim, Jeong-Ok
A1 Baek, Woon-Yi
A1 Suh, Byung-Sun
A1 Kim, Kyong-Tai
YR 2004
UL http://jpet.aspetjournals.org/content/309/2/641.abstract
AB We investigated the effect of the phytoestrogen cimicifugoside, one of the pharmacologically active ingredients of the medicinal plant Cimicifuga racemosa (black cohosh) that has been used to treat many kinds of neuronal and menopausal symptoms, such as arthritis, menopausal depression, and nerve pain. Cimicifugoside inhibited calcium increase induced by 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP), a nicotinic acetylcholine receptor (nAChR) agonist in bovine adrenal chromaffin cells with a half-maximal inhibitory concentration (IC50) of 18 ± 2 μM. In contrast, cimicifugoside did not affect the calcium increases evoked by high K+, veratridine, and bradykinin. The DMPP-induced sodium increase was also inhibited by cimicifugoside with an IC50 of 2 ± 0.3 μM, suggesting that the activity of nAChRs is inhibited by cimicifugoside. Cimicifugoside did not affect the KCl-induced secretion but markedly inhibited the DMPP-induced catecholamine secretion that was monitored by carbon-fiber amperometry in real time and high-performance liquid chromatography through electrochemical detection. The results suggest that cimicifugoside selectively inhibits nAChR-mediated response in bovine chromaffin cells. The American Society for Pharmacology and Experimental Therapeutics