PT  - JOURNAL ARTICLE
AU  - Woo, Kyung-Chul
AU  - Park, Yong-Soo
AU  - Jun, Dong-Jae
AU  - Lim, Jeong-Ok
AU  - Baek, Woon-Yi
AU  - Suh, Byung-Sun
AU  - Kim, Kyong-Tai
TI  - Phytoestrogen Cimicifugoside-Mediated Inhibition of Catecholamine Secretion by Blocking Nicotinic Acetylcholine Receptor in Bovine Adrenal Chromaffin Cells
AID  - 10.1124/jpet.103.062331
DP  - 2004 May 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 641--649
VI  - 309
IP  - 2
4099  - http://jpet.aspetjournals.org/content/309/2/641.short
4100  - http://jpet.aspetjournals.org/content/309/2/641.full
SO  - J Pharmacol Exp Ther2004 May 01; 309
AB  - We investigated the effect of the phytoestrogen cimicifugoside, one of the pharmacologically active ingredients of the medicinal plant Cimicifuga racemosa (black cohosh) that has been used to treat many kinds of neuronal and menopausal symptoms, such as arthritis, menopausal depression, and nerve pain. Cimicifugoside inhibited calcium increase induced by 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP), a nicotinic acetylcholine receptor (nAChR) agonist in bovine adrenal chromaffin cells with a half-maximal inhibitory concentration (IC50) of 18 ± 2 μM. In contrast, cimicifugoside did not affect the calcium increases evoked by high K+, veratridine, and bradykinin. The DMPP-induced sodium increase was also inhibited by cimicifugoside with an IC50 of 2 ± 0.3 μM, suggesting that the activity of nAChRs is inhibited by cimicifugoside. Cimicifugoside did not affect the KCl-induced secretion but markedly inhibited the DMPP-induced catecholamine secretion that was monitored by carbon-fiber amperometry in real time and high-performance liquid chromatography through electrochemical detection. The results suggest that cimicifugoside selectively inhibits nAChR-mediated response in bovine chromaffin cells. The American Society for Pharmacology and Experimental Therapeutics