PT  - JOURNAL ARTICLE
AU  - Armin Kabat
AU  - Klaus Pönicke
AU  - Aida Salameh
AU  - Friedrich-Wilhelm Mohr
AU  - Stefan Dhein
TI  - Effect of a β<sub>2</sub>-Adrenoceptor Stimulation on Hyperglycemia-Induced Endothelial Dysfunction
AID  - 10.1124/jpet.103.057554
DP  - 2004 Feb 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 564--573
VI  - 308
IP  - 2
4099  - http://jpet.aspetjournals.org/content/308/2/564.short
4100  - http://jpet.aspetjournals.org/content/308/2/564.full
SO  - J Pharmacol Exp Ther2004 Feb 01; 308
AB  - To investigate whether β2-adrenoceptors exist on endothelial cells and whether a β2-adrenoceptor stimulation might prevent the development of hyperglycemia-induced endothelial dysfunction, porcine aortic endothelial cells (PAECs) were cultured and chronically exposed to either 5 mM d-glucose (“normoglycemia”) or 20 mM d-glucose (“hyperglycemia”), with or without 100 nM salbutamol in absence or presence of β2-adrenoceptor antagonist ICI 118,551 [1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxyl]-3-[(1-methylethyl)-amino]-2-butanol] or β1-antagonist metoprolol. For osmotic control, PAECs were exposed to 15 mM l-glucose. We measured nitric oxide release using the met-hemoglobin assay and assessed β-adrenoceptor density and subtypes by radioligand binding. Furthermore, we determined intracellular NADH and NADPH using high-performance liquid chromatography. High d-glucose concentrations but not l-glucose led to significantly reduced basal and stimulated nitric oxide release. Chronic salbutamol treatment significantly antagonized the impairment of the nitric oxide response, which was inhibited by ICI 118,551 but not by metoprolol. The number of giant cells was significantly increased in hyperglycemia, which could be prevented by salbutamol. Binding of the radioligand (–)-[125I]iodocyanopindolol revealed a total β-adrenoceptor density of 29.8 ± 3.7 (normoglycemic) and 30.3 ± 3.6 (hyperglycemic) fmol/mg protein. Displacement by ICI 118,551 revealed β-adrenoceptor subtype distribution with 30.3 ± 4.4 (normoglycemic) and 29.1 ± 3.8% β2-adrenoceptors. NADH production increased in hyperglycemia, which was completely prevented by salbutamol. We conclude that hyperglycemia in PAEC induces endothelial dysfunction with impaired nitric oxide release and that this can be prevented by β2-adrenoceptor stimulation. The American Society for Pharmacology and Experimental Therapeutics