TY - JOUR T1 - Kinin-Induced Anion-Dependent Secretion in Porcine Ileum: Characterization and Involvement of Opioid- and Cannabinoid-Sensitive Enteric Neural Circuits JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 733 LP - 739 DO - 10.1124/jpet.102.047829 VL - 305 IS - 2 AU - Benedict T. Green AU - Andrew Calvin AU - Scott M. O'Grady AU - David R. Brown Y1 - 2003/05/01 UR - http://jpet.aspetjournals.org/content/305/2/733.abstract N2 - The intestinal secretory actions of the proinflammatory peptide kallidin (lysyl-bradykinin) are mediated partially by enteric neurons. We hypothesized that kallidin produces neurogenic anion secretion through opioid- and cannabinoid-sensitive enteric neural pathways. Changes in short-circuit current (Isc) across sheets of porcine ileal mucosa-submucosa mounted in Ussing chambers were measured in response to kallidin (1 μM) or drugs added to the contraluminal bathing medium. Kallidin transiently increased Isc, an effect reduced after inhibition of neuronal conduction by 0.1 μM saxitoxin, cyclooxygenase inhibition by 10 μM indomethacin, or kinin B2 receptor blockade by 1 μMd-arginyl-l-arginyl-l-prolyl-trans-4-hydroxy-l-prolylglycyl-3-(2-thienyl)-l-alanyl-l-seryl-d-1,2,3,4-tetrahydro-3-isoquinolinecarbonyl-l-(2α,3β,7αβ)-octahydro-1H-indole-2-carbonyl-l-arginine (HOE-140). Its action was dependent upon extracellular Cl−or HCO 3− ions, but was resistant to 10 μM bumetanide or 0.3 mM 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid, and seemed to involve luminal alkalinization as measured by pH-stat titration. Kallidin-induced Isc elevations were sensitive to saxitoxin in tissues bathed in Cl−-, but not HCO 3− -deficient media. Tissues pretreated with 0.1 μM [d-Pen2,5]-enkephalin, a selective δ-opioid agonist, displayed reduced Isc responses to kallidin; this effect was prevented by the δ-opioid antagonist naltrindole. At a contraluminal concentration of 1 μM, the cannabinoid receptor agonist (6aR)-trans-3-(1,1-dimethylheptyl)-6a,7,10,10a-tetrahydro-1-hydroxy-6,6-dimethyl-6H-dibenzo[b,d]pyran-9-methanol (HU-210) also attenuated responses to kallidin. Proinflammatory kinins seem to stimulate neurogenic anion secretion in porcine ileum by activating enteric neural circuits expressing inhibitory opioid and possibly cannabinoid receptors. The American Society for Pharmacology and Experimental Therapeutics ER -