PT - JOURNAL ARTICLE AU - Atsushi Masamune AU - Masahiro Satoh AU - Kazuhiro Kikuta AU - Yoshitaka Sakai AU - Akihiko Satoh AU - Tooru Shimosegawa TI - Inhibition of p38 Mitogen-Activated Protein Kinase Blocks Activation of Rat Pancreatic Stellate Cells AID - 10.1124/jpet.102.040287 DP - 2003 Jan 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 8--14 VI - 304 IP - 1 4099 - http://jpet.aspetjournals.org/content/304/1/8.short 4100 - http://jpet.aspetjournals.org/content/304/1/8.full SO - J Pharmacol Exp Ther2003 Jan 01; 304 AB - Activated pancreatic stellate cells (PSCs) have recently been implicated in the pathogenesis of pancreatic fibrosis and inflammation. However, the signal transduction pathways in PSCs remain largely unknown. We examined the role of p38 mitogen-activated protein (MAP) kinase in the activation of PSCs. PSCs were isolated from rat pancreas tissue and used in their culture-activated, myofibroblast-like phenotype. Activation of p38 MAP kinase was determined by Western blotting using anti-phosphospecific antibody. The effects of two p38 MAP kinase inhibitors, 4-(4-flurophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)imidazole (SB203580) and 4-(4-flurophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)1H-imidazole (SB202190), on the parameters of PSC activation, including proliferation, expression of α-smooth muscle actin, α1(I) procollagen, and prolyl 4-hydroxylase (α) genes, and monocyte chemoattractant protein-1 production were evaluated. Interleukin-1β and platelet-derived growth factor-BB activated p38 MAP kinase. Platelet-derived growth factor-induced PSC proliferation was inhibited by SB203580 and SB202190. These reagents decreased α-smooth muscle actin protein expression, and α1(I) procollagen and prolyl 4-hydroxylase (α) mRNA levels. Treatment with these p38 MAP kinase inhibitors also resulted in inhibition of monocyte chemoattractant protein-1 expression. In addition, SB203580 inhibited spontaneous activation of freshly isolated PSCs in culture on plastic. Thus, inhibition of p38 MAP kinase modulated profibrogenic and proinflammatory actions in PSCs, implying a potential application of p38 MAP kinase inhibitors for the treatment of pancreatic fibrosis and inflammation. The American Society for Pharmacology and Experimental Therapeutics