PT  - JOURNAL ARTICLE
AU  - Jeffrey M. Brown
AU  - Evan L. Riddle
AU  - Verónica Sandoval
AU  - Raul K. Weston
AU  - Jarom E. Hanson
AU  - Michael J. Crosby
AU  - Yvette V. Ugarte
AU  - James W. Gibb
AU  - Glen R. Hanson
AU  - Annette E. Fleckenstein
TI  - A Single Methamphetamine Administration Rapidly Decreases Vesicular Dopamine Uptake
AID  - 10.1124/jpet.302.2.497
DP  - 2002 Aug 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 497--501
VI  - 302
IP  - 2
4099  - http://jpet.aspetjournals.org/content/302/2/497.short
4100  - http://jpet.aspetjournals.org/content/302/2/497.full
SO  - J Pharmacol Exp Ther2002 Aug 01; 302
AB  - Recent studies demonstrated that vesicular dopamine (DA) uptake can be rapidly altered in synaptic vesicles purified from the striata of stimulant-treated rats. Specifically, a single administration of the plasmalemmal DA transporter inhibitor, cocaine, or the DA D2 agonist, quinpirole, increases vesicular DA uptake in vesicles purified from the striata of treated rats. These effects of cocaine are prevented by pretreatment with a D2, but not D1, DA receptor antagonist. The purpose of the present study was to characterize the effect of a mechanistically different psychostimulant, methamphetamine (METH), on vesicular DA uptake. Results demonstrated that a single administration of this DA-releasing agent rapidly and reversibly decreased vesicular DA uptake. The METH-related decrease in vesicular DA uptake was attenuated by pretreatment with the D2 antagonist, eticlopride, but not the D1 antagonist, SCH23390 (R-[+]-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine). Core body temperature did not contribute to the effects of METH on vesicular DA uptake. Neither quinpirole nor cocaine increased vesicular DA uptake when rats were concurrently treated with METH. These studies provide further evidence that psychostimulants rapidly and differentially modify vesicular DA uptake. In addition, these studies demonstrate a complex role for D2 DA receptors in altering vesicular DA transport. The American Society for Pharmacology and Experimental Therapeutics