TY - JOUR T1 - Nicotine Increases Hepatic Oxygen Uptake in the Isolated Perfused Rat Liver by Inhibiting Glycolysis JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 930 LP - 937 DO - 10.1124/jpet.301.3.930 VL - 301 IS - 3 AU - Brian J. Dewar AU - Blair U. Bradford AU - Ronald G. Thurman Y1 - 2002/06/01 UR - http://jpet.aspetjournals.org/content/301/3/930.abstract N2 - Nicotine influences energy metabolism, yet mechanisms remain unclear. Since the liver is one of the largest organs and performs many metabolic functions, the goal of this study was to determine whether nicotine would affect respiration and other metabolic functions in the isolated perfused liver. Infusion of 85 μM nicotine caused a rapid 10% increase in oxygen uptake over basal values of 105 ± 5 μmol/g/h in perfused livers from fed rats, and an increase of 27% was observed with 850 μM nicotine. Concomitantly, rates of glycolysis of 105 ± 8 μmol/g/h were decreased to 52 ± 9 μmol/g/h with nicotine, whereas ketone body production was unaffected. Nicotine had no effect on oxygen uptake in glycogen-depleted livers from 24-h fasted rats. Furthermore, addition of glucose to perfused livers from fasted rats partially restored the stimulatory effect of nicotine. Infusion of atractyloside, potassium cyanide, or glucagon blocked the nicotine-induced increase in respiration. Intracellular calcium was increased in isolated hepatocytes by nicotine, a phenomenon prevented by incubation of cells with d-tubocurarine, a nicotinic acetylcholine receptor antagonist. Respiration was also increased ∼30% in hepatocytes isolated from fed rats by nicotine, whereas hepatocytes isolated from fasted rats showed little response. In the presence ofN-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), an inhibitor of cyclic AMP-dependent protein kinase A, nicotine failed to stimulate respiration. These data support the hypothesis that inhibition of glycolysis by nicotine increases oxygen uptake due to an ADP-dependent increase in mitochondrial respiration. The American Society for Pharmacology and Experimental Therapeutics ER -