PT - JOURNAL ARTICLE AU - Hiroko Matsumoto AU - Akira Fujii TI - Tenidap, an Anti-Inflammatory Agent, Inhibits DNA and Collagen Syntheses, Depresses Cell Proliferation, and Lowers Intracellular pH in Cultured Human Gingival Fibroblasts AID - 10.1124/jpet.300.2.668 DP - 2002 Feb 01 TA - Journal of Pharmacology and Experimental Therapeutics PG - 668--672 VI - 300 IP - 2 4099 - http://jpet.aspetjournals.org/content/300/2/668.short 4100 - http://jpet.aspetjournals.org/content/300/2/668.full SO - J Pharmacol Exp Ther2002 Feb 01; 300 AB - The effect of tenidap [(±)-5-chloro-2,3-dihydro-3-(hydroxy-2-thienylmethylene)-2-oxo-1H-indole-1-carboxamide], a new anti-inflammatory agent, was investigated on DNA synthesis by means of [3H]thymidine incorporation, collagen synthesis by means of [3H]proline incorporation, cell proliferation, and intracellular pH in nicardipine-reactive human gingival fibroblasts. Tenidap significantly inhibited [3H]thymidine incorporation at concentrations greater than 20 μM on the 4th and 8th day of treatment. Tenidap also significantly inhibited [3H]proline incorporation at a concentration greater than 50 μM on the 4th day and at more than 20 μM on the 8th day of treatment. The presence of 1 μM nifedipine or 1 μM nicardipine did not alter the depressing effect of tenidap. Tenidap (20 μM) also lowered intracellular pH. These results suggest that tenidap might be effective for the prevention of gingival overgrowth caused by calcium channel blockers. The American Society for Pharmacology and Experimental Therapeutics