RT Journal Article
SR Electronic
T1 The Effects of Cocaine and Nicotine on Amino Acid Transport across the Human Placental Cotyledon Perfused In Vitro
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 141
OP 146
VO 294
IS 1
A1 Aleksandra Pastrakuljic
A1 Lidia O. Derewlany
A1 Brenda Knie
A1 Gideon Koren
YR 2000
UL http://jpet.aspetjournals.org/content/294/1/141.abstract
AB The inhibitory effects of cocaine and nicotine on placental amino acid transport, as a mechanism contributing to intrauterine growth restriction, were investigated in the in vitro placental perfusion model. Amino acids that represent substrates for known placental transporters were selected: alanine (system A), glutamine (system N), phenylalanine and valine (system l), and arginine (system y+). Amino acid accumulation on the fetal side was measured in the absence of cocaine or nicotine (n = 7) and in the presence of 1.2 μg/ml cocaine (n = 6), 120 ng/ml nicotine (n = 6), or both (n = 6). Neither cocaine nor nicotine alone significantly inhibited alanine transport, whereas their combination did (P = .02). Significant inhibition of arginine transport was detected with nicotine (P = .007), cocaine (P = .01), and their combination (P = .01), whereas phenylalanine (P = .03, P = .04) and valine (P = .03, P = .04) transport was affected by cocaine and the combination of cocaine and nicotine, respectively. For glutamine, neither cocaine, nicotine, nor their combination had a statistically significant inhibitory effect. In conclusion, both cocaine and nicotine may contribute to fetal growth restriction by interfering with the activity of amino acids transporters that are necessary to maintain the nutrient gradients associated with normal fetal growth. The American Society for Pharmacology and Experimental Therapeutics