RT Journal Article
SR Electronic
T1 Zinc Stimulates DNA Synthesis during Its Antiapoptotic Action Independently with Increments of an Antiapoptotic Protein, Bcl-2, in Porcine Kidney LLC-PK<sub>1</sub> Cells
JF Journal of Pharmacology and Experimental Therapeutics
JO J Pharmacol Exp Ther
FD American Society for Pharmacology and Experimental Therapeutics
SP 923
OP 928
VO 290
IS 2
A1 Masami Ishido
A1 Tsuguyoshi Suzuki
A1 Tatsumi Adachi
A1 Manabu Kunimoto
YR 1999
UL http://jpet.aspetjournals.org/content/290/2/923.abstract
AB Cadmium, an environmental pollutant, caused nephroptosis that was inhibitable by zinc. The mechanism of the antiapoptotic action of zinc is poorly understood. In this study, we found the stimulation of DNA synthesis, as assessed by bromodeoxyuridine incorporation, during prevention by zinc of apoptosis, suggesting that the proliferactive nature of zinc contributes to its inhibition of apoptosis. This finding was consistent with the result that the cells driven by dialyzed fetal bovine serum were resistant to apoptotic stimuli of cadmium. Furthermore, zinc activated the expression of endogenous Bcl-2 proteins. However, overexpression of Bcl-2 proteins by transfection did not facilitate zinc-mediated DNA synthesis. Thus, one possible role of zinc in the prevention of apoptosis is to promote DNA synthesis independently with activation of antiapoptotic proteins Bcl-2. The American Society for Pharmacology and Experimental Therapeutics