PT  - JOURNAL ARTICLE
AU  - Junya Nagai
AU  - Ikuko Yano
AU  - Yukiya Hashimoto
AU  - Mikihisa Takano
AU  - Ken-Ichi Inui
TI  - Efflux of Intracellular α-Ketoglutarate Via p-Aminohippurate/Dicarboxylate Exchange in OK Kidney Epithelial Cells
DP  - 1998 May 01
TA  - Journal of Pharmacology and Experimental Therapeutics
PG  - 422--427
VI  - 285
IP  - 2
4099  - http://jpet.aspetjournals.org/content/285/2/422.short
4100  - http://jpet.aspetjournals.org/content/285/2/422.full
SO  - J Pharmacol Exp Ther1998 May 01; 285
AB  - The involvement of intracellular α-ketoglutarate (α-KG) in p-aminohippurate (PAH) transport was investigated in OK kidney epithelial cells. Efflux of intracellular α-KG from the OK cells to the basolateral side was increased by applying PAH to the basolateral side of the cells. In contrast, the intracellular α-KG concentration was not influenced by the addition of PAH. The α-KG efflux across the basolateral membrane induced by PAH was higher than that across the apical membrane. Probenecid inhibited the PAH-dependent α-KG efflux. The α-KG efflux to the basolateral side was saturable with increasing concentration of PAH in the basolateral medium. Antimycin A, a metabolic inhibitor, inhibited [14C]PAH uptake across the basolateral membrane of OK cells in a dose-dependent manner. In addition, both the α-KG efflux induced by PAH and the intracellular α-KG concentration were decreased by antimycin A dose-dependently. These results directly show that α-KG generated by intracellular metabolism is effluxed via PAH/dicarboxylate exchange in the basolateral membrane of OK cells. The American Society for Pharmacology and Experimental Therapeutics