RT Journal Article SR Electronic T1 Leukotriene D4 Contractions in Human Airways are Blocked by SK&F 96365, an Inhibitor of Receptor-Mediated Calcium Entry JF Journal of Pharmacology and Experimental Therapeutics JO J Pharmacol Exp Ther FD American Society for Pharmacology and Experimental Therapeutics SP 549 OP 552 VO 284 IS 2 A1 Isabelle Gorenne A1 Carlos Labat A1 Jean-Pierre Gascard A1 Xavier Norel A1 Nabila Nashashibi A1 Charles Brink YR 1998 UL http://jpet.aspetjournals.org/content/284/2/549.abstract AB In human bronchial muscle preparations, nifedipine (3 μM) significantly inhibited the histamine, ACh and KCl contractions. However, the dihydropyridine did not modify the contractile responses induced by either leukotriene D4 (LTD4) or anti-human IgE (a-IgE). In human airways, SK&F 96365 (30 μM and 100 μM) markedly reduced the KCl and, at the higher concentration, LTD4 maximal contractions. In addition, when preparations were treated with nifedipine (3 μM), SK&F 96365 (100 μM) significantly blocked responses to both LTD4 and a-IgE. The calcium chelating agent ethylene glycol-bis (β-amino-ethyl ether) N,N,N′,N′-tetraacetic acid (4 mM) also inhibited the a-IgE-induced contractions. These data demonstrate that the nifedipine-resistant component of the LTD4 and a-IgE contractions was inhibited by SK&F 96365 and suggest that the cysteinyl-leukotriene receptor in human airways may be intimately linked with a receptor-operated calcium-entry mechanism. The American Society for Pharmacology and Experimental Therapeutics