TY - JOUR T1 - Selective Actions of a Detergent on Ligand-Gated Ion Channels Expressed in <em>Xenopus</em> Oocytes JF - Journal of Pharmacology and Experimental Therapeutics JO - J Pharmacol Exp Ther SP - 32 LP - 36 VL - 284 IS - 1 AU - Tina K. Machu AU - S. John Mihic AU - J. E. Dildy-Mayfield Y1 - 1998/01/01 UR - http://jpet.aspetjournals.org/content/284/1/32.abstract N2 - Cytoclean, a commercially available detergent, has selective actions on ligand-gated ion channels. Cytoclean (0.0005–0.01% v/v) potentiated 50 μM glycine responses in oocytes expressingalpha-2 glycine receptors by 23 ± 7% to 342 ± 43%. Cytoclean is composed of five components dissolved in water, but only one reagent, Bio-Soft D-62, modulated responses of oocytes expressing alpha-2 glycine receptors. Bio-Soft D-62 (0.00005–0.001% w/v), potentiated 50 μM glycine responses by 13 ± 1% to 474 ± 50%. Bio-Soft D-62 is composed of linear alkylbenzene sulfonate (&gt;95% C12 chain). The effects of Cytoclean or Bio-Soft D-62 were examined on alpha-1beta-2 and alpha-1 beta-2gamma-2L γ-aminobutyric acidA, γ-aminobutyric acidρ1,dl-α-amino-3-hydroxy-5-methyl-4-isoxalonepropionic acid, kainate and 5- hydroxytryptamine3 receptors expressed inXenopus laevis oocytes. Enhancement of γ-aminobutyric acidA receptor function ranged from ≈21% to 458% with Cytoclean (0.0001–0.01%), respectively. Bio-Soft D-62 (0.001%) inhibited GABAρ1 receptor function by ≈72%. Cytoclean had no effect on 5- hydroxytryptamine3 or GluR6 function, but Cytoclean (0.005% and 0.01%) inhibited GluR3-mediated currents by ≈21% and ≈41%, respectively. These results suggest that trace amounts of Cytoclean, such as amounts adhering to glassware, may modulate ion channel function and potentially confound experimental results. The American Society for Pharmacology and Experimental Therapeutics ER -